protein 20 extraction kit  (Invent Biotechnologies)

Journal: bioRxiv

Article Title: Identification of the Park Grass Experiment soil metaproteome

doi: 10.1101/2021.10.25.465615

Figure Lengend Snippet: Community composition of Park Grass soil microbiome (metagenome and metaproteome). Phyla ratios derived from 976,268 sequences from the original soil metagenome (metasoil F1, Feb 2009) and 1488 normalised weighted spectra for both the HTPC and surfactant methods. The proteomic data is based on the homogenised PGE soil sample extracted in triplicate by the HTPC and surfactant protein extraction methods.

Article Snippet: There are other soil protein extraction techniques which operate on a purely physical basis such as freeze/thaw techniques ( ) and others which are not known such as commercial soil protein extraction kits like NoviPure Soil Protein Kit (Qiagen).

Techniques: Derivative Assay, Protein Extraction

Journal: bioRxiv

Article Title: Identification of the Park Grass Experiment soil metaproteome

doi: 10.1101/2021.10.25.465615

Figure Lengend Snippet: Protein function quantification in Park Grass experiment soil. Quantification of protein function by normalised values (NSAF) based on the identification of 715 proteins by surfactant extraction and 635 proteins by HTPC extraction. The segments of the outer rings represent low stringency protein identification, the inside segments of the circles represent high stringency protein identification. Data based on the homogenised Park Grass soil sample extracted in triplicate by HTPC and surfactant protein extraction methods. Annotation based on level 1 SEED categorisation by MG-RAST.

Article Snippet: There are other soil protein extraction techniques which operate on a purely physical basis such as freeze/thaw techniques ( ) and others which are not known such as commercial soil protein extraction kits like NoviPure Soil Protein Kit (Qiagen).

Techniques: Extraction, Protein Extraction

Journal: bioRxiv

Article Title: Identification of the Park Grass Experiment soil metaproteome

doi: 10.1101/2021.10.25.465615

Figure Lengend Snippet: Comparison of protein extraction methods from Park Grass Experiment soil. Bland-Altman plot of (a) low stringency protein identification (i.e., each protein identified by one peptide). Data averaged over the three replicates for surfactant and HTPC, respectively. A total of 631 proteins were identified by surfactant but not by HTPC (dots at +200%), while 551 proteins were identified by HTPC but not by surfactant (dots at -200%). Bland-Altman plot of (b) high stringency protein identification (i.e., each protein identified by at least two peptides). Data averaged over the three replicates for surfactant and HTPC respectively. A total of 66 proteins were identified by surfactant but not by HTPC (dots at +200%), while 104 proteins were identified by HTPC but not by surfactant (dots at -200%).

Article Snippet: There are other soil protein extraction techniques which operate on a purely physical basis such as freeze/thaw techniques ( ) and others which are not known such as commercial soil protein extraction kits like NoviPure Soil Protein Kit (Qiagen).

Techniques: Comparison, Protein Extraction